Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)((py36.bat and py35.sh)
5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory.
6. Download [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it into knime-workspace directory.
6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
#### Structure of workflow
- two user inputs:
- main user input
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@@ -59,12 +61,11 @@ Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-v
4. Execute all nodes or execute different parts of pipeline sequentially
> For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
5. Delete point that you consider have "bad" fluctuations of fitting (list points to delete in plot parameter input.
#### Interactive Visualisation of Calibration Plot
1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS position.
2. Check the statistics parameters and level of bleaching at the headings of plots.
2. The menu of the plot windows can be used to:
3. Delete point that you consider have "bad" fluctuations of fitting (list points to delete in plot parameter input.