Update Home authored by Vitali Vistunou's avatar Vitali Vistunou
...@@ -7,11 +7,13 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC ...@@ -7,11 +7,13 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
#### 4. [Interactive Visualisation of Calibration Plot](#interactive-visualisation-of-calibration-plot) #### 4. [Interactive Visualisation of Calibration Plot](#interactive-visualisation-of-calibration-plot)
#### 5. [Output files](#output-files) #### 5. [Output files](#output-files)
#### Installation #### Installation
1. Install [Anaconda](https://www.anaconda.com/distribution/) with Python3. 1. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth.
2. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X. > In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
2. Install [Anaconda](https://www.anaconda.com/distribution/) with Python3.
3. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X.
> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform. > Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
3. Install KNIME Image Processing extension (File -> Install KNIME Extensions). 4. Install KNIME Image Processing extension (File -> Install KNIME Extensions).
4. Install the KNIME Python Integration extension (File -> Install KNIME Extensions). 5. Install the KNIME Python Integration extension (File -> Install KNIME Extensions).
Follow these steps as described in [KNIME Python Integration Installation Guide](https://docs.knime.com/2018-12/python_installation_guide/index.html): Follow these steps as described in [KNIME Python Integration Installation Guide](https://docs.knime.com/2018-12/python_installation_guide/index.html):
* Download a configuration file [fcspy.yml](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) * Download a configuration file [fcspy.yml](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)
...@@ -31,9 +33,9 @@ CALL activate py36_knime || ECHO Activating python environment failed ...@@ -31,9 +33,9 @@ CALL activate py36_knime || ECHO Activating python environment failed
``` ```
Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh) Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh)
5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory. 6. Download [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it into knime-workspace directory.
6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
#### Structure of workflow #### Structure of workflow
- two user inputs: - two user inputs:
- main user input - main user input
...@@ -59,12 +61,11 @@ Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-v ...@@ -59,12 +61,11 @@ Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-v
4. Execute all nodes or execute different parts of pipeline sequentially 4. Execute all nodes or execute different parts of pipeline sequentially
> For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7. > For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
5. Delete point that you consider have "bad" fluctuations of fitting (list points to delete in plot parameter input.
#### Interactive Visualisation of Calibration Plot #### Interactive Visualisation of Calibration Plot
1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS position. 1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS position.
2. Check the statistics parameters and level of bleaching at the headings of plots. 2. Check the statistics parameters and level of bleaching at the headings of plots.
2. The menu of the plot windows can be used to: 3. Delete point that you consider have "bad" fluctuations of fitting (list points to delete in plot parameter input.
4. The menu of the plot windows can be used to:
* Save the image of the plot * Save the image of the plot
* Move and zoom a working space * Move and zoom a working space
* Adjust spacing and export values from the plot * Adjust spacing and export values from the plot
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