@@ -30,7 +30,7 @@ The WT processing block calculates the background offset for the correction step
#### II - main user input
The main user input contains the data input path and general parameters of the FCSpipelineEMBL_KNIME workflow. The explanation of every parameter can be found on the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page or in a KMIME description menu of the main user input.
The main user input contains the general parameters of the FCSpipelineEMBL_KNIME workflow. The explanation of every parameter can be found on the [input parameters](https://git.embl.de/grp-almf/FCSpipelineEMBL_KNIME/-/wikis/input-parameters) page or in a KMIME description menu of the main user input.
#### III - plot parameters input
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@@ -38,7 +38,7 @@ The plot parameters input contains a calibration plot and QC parameters. For par
#### IV - visualization block
The block provides visualization of FCSpipelineEMBL_KNIME outputs, inspecting the results and saving them in output folder.
The block provides visualization of FCSpipelineEMBL_KNIME outputs, inspecting the results and saving them in the output folder.
# Procedure
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@@ -50,7 +50,7 @@ The block provides visualization of FCSpipelineEMBL_KNIME outputs, inspecting th
- Click FA format to save corresponding res file as **WT.res**
- Fill the WT user input and execute Python Source node. Use returned offset value (right-click -> Table) for correction steps in further FA sessions for FP and POI.
- Fill the WT user input and execute Python Source node. Use returned offset value (right-click -> Table) for correction steps in further FA sessions for FP&POI.
#### 2. Perform 2 separate FA analysis sessions on FCS data for **POI** and **FP**:
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@@ -103,7 +103,7 @@ For the execution of all nodes at one time, press a shortcut: Shift+F7. Descript
3) See the estimated concentrations of POI via FCS-calibrated imaging in the output table of **concentrations of POI** node.
4) Knime has his own Image viewer. To see raw images with concentration maps, user can right-click the Image Viewer nodes and go to View: Image Viewer. Then, double-click the image of interest.
4) Knime has his own Image viewer. To see raw images with concentration maps, users can right-click the Image Viewer nodes and go to View: Image Viewer. Then, double-click the image of interest.
