Update Home authored by Vitali Vistunou's avatar Vitali Vistunou
Welcome to a Wiki page of FCS pipeline! ### Welcome to a Wiki page of FCS pipeline!
1. [Installation](Installation). #### 1. [Installation](Installation)
2. Prepare the following [structure of directories](structure of directories) with the data from FCS experiment. #### 2. Prepare the following [structure of directories](structure of directories) which contains:
3. ![fcs_pipeline workflow](uploads/2c5fb65b19810368bcee452f8d6ea874/workflow.png) * Output data from FCS experiments: .czi, .fcs, .row files produced by [ZEN software](https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html)
\ No newline at end of file * Output files from [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth
#### 3. Structure of workflow:
- two user inputs:
- main user input
- plot parameters input
- loading metanodes:
- collecting fluctuation data (FA WT, FA FP, FA dye metanodes)
- collecting intensities (intensity data metanode)
- data processing nodes (joiner, python scripts)
- visualisation node (Python View)
![fcs_pipeline workflow](uploads/2c5fb65b19810368bcee452f8d6ea874/workflow.png)
#### 4. Prosedure:
1. Fill main user input: