> For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
#### Interactive Visualisation of Calibration Plot
1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from respective FCS position.
1. Pick the point of interest in the calibration plot window to see fluctuation and correlation data from the respective FCS position.
2. Check the statistics parameters and level of bleaching at the headings of plots.
3. Delete point that you consider have "bad" fluctuations of fitting (list points to delete in plot parameter input.
3. Delete points that you consider have "bad" fluctuations or quality of fitting (list points to delete in plot parameter input).
4. The menu of the plot windows can be used to:
* Save the image of the plot
* Move and zoom a working space
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@@ -82,11 +85,10 @@ info.csv includes:
* the names of directories and files with FCS data
* path to the main directory
* calibration plot parameters and its errors
* k - parameter that shows the intersection rate of intensity ranges of POI and FP
2.**calibration.csv**
Here you can find the concentrations of POI calculated using calibration plot. Check the k-value in the info.csv after execution. k > 1 leads to less confidence in the determination of POI concentration.
Here you can find the concentrations of POI calculated using calibration plot.