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...@@ -6,33 +6,6 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC ...@@ -6,33 +6,6 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
#### 3. [Procedure](#procedure) #### 3. [Procedure](#procedure)
#### 4. [Interactive Visualisation](#interactive-visualisation-of-calibration-plot) #### 4. [Interactive Visualisation](#interactive-visualisation-of-calibration-plot)
#### 5. [Output files](#output-files) #### 5. [Output files](#output-files)
#### Installation
1. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth.
> In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
2. Install [Anaconda](https://www.anaconda.com/distribution/) with Python3.
3. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X.
> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
4. Install KNIME Image Processing extension (File -> Install KNIME Extensions).
5. Install the KNIME Python Integration extension (File -> Install KNIME Extensions).
Follow these steps as described in [KNIME Python Integration Installation Guide](https://docs.knime.com/2018-12/python_installation_guide/index.html):
* Download a configuration file [fcspy.yml](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)
* Type in your terminal (for Windows use command prompt or anaconda prompt)
```
conda env create -f <PATH-TO-FOLDER-WITH-fcspy.yml>/fcspy.yml
```
* For Windows user: create py36.bat that will contain the path to your Anaconda folder (**this Anaconda folder must contain Scripts folder**):
```
@REM Adapt the folder in the PATH to your system
SET PATH=<PATH_TO_ANACONDA_FOLDER>\Scripts;%PATH%
CALL activate py36_knime || ECHO Activating python environment failed
@python %*
```
* For Linux users: create py36.sh with the path to your Anaconda folder (**must contain bin folder**):
6. Download [the last version of FCSpipeline](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it into the knime-workspace directory where all KNIME workflows are stored.
#### Structure of workflow #### Structure of workflow
- three user inputs: - three user inputs:
- main user input (general parameters) - main user input (general parameters)
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