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@@ -49,7 +49,7 @@ CALL activate py36_knime || ECHO Activating python environment failed
 #### Procedure 
 1.  Prepare the following [structure of files](structure of files)
 2.  Specify parameters in a main user input
-> You can change any parameters or leave default values. Explanation of every field can be founded in a description menu of KNIME.
+> You can change any parameters or leave default values. The explanation of every parameter can be founded in a description menu of KNIME node.
 
 ![input1](uploads/a4c9df72fe2acfc0693cc9b274e0fba6/input1.png)
 
@@ -58,7 +58,9 @@ CALL activate py36_knime || ECHO Activating python environment failed
 
 ![input6](uploads/9722c2ef7708c222886222c7f15ad864/input6.png)
 
-4.  Execute all nodes or execute different parts of pipeline sequentially
+4. The default channel used for extracting intensities from images is the first one. If you want to change the channel, go to intensity data metanode, then open Image Reader (Table) node, go to Subset Selection and exclude all channels except the channel you need to process. 
+
+5.  Execute all nodes or execute different parts of pipeline sequentially
 > For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
 
 #### Interactive Visualisation of Calibration Plot