Here we explain how to install and use Fluorescence Correlation Spectroscopy (FCS) pipeline. The FCS pipeline is an automating FCS-based calibrated imaging analysis software for the interactive and automated processing of FCS data.
Here we explain how to install and use Fluorescence Correlation Spectroscopy (FCS) pipeline. The FCS pipeline is a FCS-based calibrated imaging analysis software for the interactive and automated processing of FCS data.
#### 1. [Installation](#installation)
#### 2. [Structure of workflow](#structure-of-workflow)
#### 3. [Prosedure](#prosedure)
#### 3. [Prosedure](#prosedure)
#### Installation
1. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads).
2. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform.
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* Output data from FCS experiments: .czi, .fcs, .row files produced by [ZEN software](https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html)
* Output files from [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth
