Update Home authored by Vitali Vistunou's avatar Vitali Vistunou
...@@ -10,17 +10,31 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC ...@@ -10,17 +10,31 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
1. Install [Anaconda](https://www.anaconda.com/distribution/) with Python3. 1. Install [Anaconda](https://www.anaconda.com/distribution/) with Python3.
2. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X. 2. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads). It is available for Windows, Linux, and Mac OS X.
> Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform. > Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
3. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform. 3. Install KNIME Image Processing extension (File -> Install KNIME Extensions).
4. Install the KNIME Python Integration extension (File -> Install KNIME Extensions).
Follow this steps: Follow these steps as described in [KNIME Python Integration Installation Guide](https://docs.knime.com/2018-12/python_installation_guide/index.html):
* Download a configuration file [fcspy.yml](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) * Download a configuration file [fcspy.yml](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)
* Type in your terminal (for windows command prompt or anaconda prompt) * Type in your terminal (for Windows use command prompt or anaconda prompt)
``` ```
conda env create -f <PATH-TO-FOLDER-WITH-fcspy.yml>/fcspy.yml conda env create -f <PATH-TO-FOLDER-WITH-fcspy.yml>/fcspy.yml
``` ```
conda env create -f <PATH-TO-FOLDER>/py36_knime.yml * For Windows user: create py36.bat that contains path to your Anaconda folder (**must contain Scripts folder**):
3. Save and unzip [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime-workspace directory that was created after KNIME installation. fcs_pipeline directory includes all files needed for fcs pipeline to work. ```
4. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. @REM Adapt the folder in the PATH to your system
SET PATH=<PATH_TO_ANACONDA_FOLDER>\Scripts;%PATH%
CALL activate py36_knime || ECHO Activating python environment failed
@python %*
```
* For Linux users: create py36.sh that contains path to your Anaconda folder (**must contain bin folder**):
```
```
Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh)
5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory.
> fcs_pipeline directory includes all files needed for the pipeline to work.
6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
#### Structure of workflow #### Structure of workflow
- two user inputs: - two user inputs:
- main user input - main user input
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