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Jan 24, 2020
by
Vitali Vistunou
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@@ -32,7 +32,6 @@ CALL activate py36_knime || ECHO Activating python environment failed
Examples of files are
[
here
](
https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou
)(
(py36.bat
and py35.sh)
5.
Save
[
fcs_pipeline.zip
](
https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou
)
on your computer and unzip it in knime-workspace directory.
> fcs_pipeline directory includes all files needed for the pipeline to work.
6.
Install
[
Fluctuation Analyzer (FA) software
](
http://fluctuations.de/downloads.html
)
provided by Malte Wachsmuth. In case LabVIEW error, please install
[
.NET Framework 3.5
](
https://www.microsoft.com/ru-ru/download/details.aspx?id=21
)
#### Structure of workflow
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@@ -87,8 +86,6 @@ info.csv includes:
*
calibration plot parameters and its errors
*
k - parameter that shows the intersection rate of intensity ranges of POI and FP

2.
**calibration.csv**
Here you can find the concentrations of POI calculated using calibration plot. Check the k-value in the info.csv after execution. k > 1 leads to less confidence in the determination of POI concentration.
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