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Update Home authored by Vitali Vistunou's avatar Vitali Vistunou
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...@@ -32,7 +32,6 @@ CALL activate py36_knime || ECHO Activating python environment failed ...@@ -32,7 +32,6 @@ CALL activate py36_knime || ECHO Activating python environment failed
Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh) Examples of files are [here](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou)( (py36.bat and py35.sh)
5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory. 5. Save [fcs_pipeline.zip](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) on your computer and unzip it in knime-workspace directory.
> fcs_pipeline directory includes all files needed for the pipeline to work.
6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21) 6. Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth. In case LabVIEW error, please install [.NET Framework 3.5](https://www.microsoft.com/ru-ru/download/details.aspx?id=21)
#### Structure of workflow #### Structure of workflow
...@@ -87,8 +86,6 @@ info.csv includes: ...@@ -87,8 +86,6 @@ info.csv includes:
* calibration plot parameters and its errors * calibration plot parameters and its errors
* k - parameter that shows the intersection rate of intensity ranges of POI and FP * k - parameter that shows the intersection rate of intensity ranges of POI and FP
![wh](uploads/ebd9f7de8c9c6238e062c43d6d100114/wh.png)
2. **calibration.csv** 2. **calibration.csv**
Here you can find the concentrations of POI calculated using calibration plot. Check the k-value in the info.csv after execution. k > 1 leads to less confidence in the determination of POI concentration. Here you can find the concentrations of POI calculated using calibration plot. Check the k-value in the info.csv after execution. k > 1 leads to less confidence in the determination of POI concentration.
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