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@@ -3,12 +3,14 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
 
 #### 1.  [Installation](#installation)
 #### 2.  [Structure of workflow](#structure-of-workflow)
-#### 3.  [Prosedure](#prosedure) 
+#### 3.  [Prosedure](#prosedure)
+#### 4.  [Interactive Visualisation of Calibration Plot](interactive-visualisation-of-calibration-plot)
 #### Installation
 1. Download the latest [KNIME Analytics Platform](https://www.knime.com/downloads).
 2. Install the [KNIME Python Integration](https://docs.knime.com/2018-12/python_installation_guide/index.html) to be used with KNIME Analytics Platform.
 > Take a look on [Getting Started](https://www.knime.com/knime) for a brief introduction into KNIME Analytics Platform.
-3.  Save the [FCS-pipeline workflow](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in knime workspace directory in your PC.
+3.  Save the [FCS-pipeline workflow](https://git.embl.de/halavaty/fcs-pipelines-vitali-vistunou) in KNIME workspace directory in your PC.
+4.  Install [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth.
 #### Structure of workflow
 -  two user inputs:
    -  main user input
@@ -20,13 +22,18 @@ Here we explain how to install and use Fluorescence Correlation Spectroscopy (FC
 -  visualisation node (Python View)
 ![fcs_pipeline workflow](uploads/2c5fb65b19810368bcee452f8d6ea874/workflow.png)
 #### Prosedure 
-1.  Prepare the following [structure of files](structure of files) which contains:
-*   Output data from FCS experiments: .czi, .fcs, .row files produced by [ZEN software](https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html)
-*   Output files from [Fluctuation Analyzer (FA) software](http://fluctuations.de/downloads.html) provided by Malte Wachsmuth
-2.  Fill main user input:
+1.  Prepare the following [structure of files](structure of files)
+2.  Specify parameters in a main user input
+> You can change any parameters or leave default values. Explanation of every field can be founded in a description menu of KNIME.
 
 ![input1](uploads/a4c9df72fe2acfc0693cc9b274e0fba6/input1.png)
 
-3.  Fill plot parameters input
+3.  Fill a plot parameters input   
+> It will implement a Quality Check by filtering the points for calibration plot according to the bounders of statistics parameters specified by user.
 
+![input6](uploads/9722c2ef7708c222886222c7f15ad864/input6.png)
 
+4.  Execute all nodes or execute different parts of pipeline sequentially
+> For the execution of all nodes at one time, click on the green bottom (Execute all executable nodes) at the top of the KNIME window or just press a shortcut: Shift+F7.
+
+#### Interactive Visualisation of Calibration Plot